Quality control 3: Dot blot to monitor coupling of ligands to TriCEPS. Detection by Streptavidin-HRP (horseradish peroxidase) that binds to the visualization part of TriCEPS. TriCEPS itself does not bind to the nitrocellulose membrane (see glycine control).
Upper lane: positive control INS coupled to TriCEPS
Middle lane: ligand of interest (anti-EGFR antibody) coupled to TriCEPS
Lower lane: negative control for the dot blot, glycine coupled to TriCEPS
Dilutions as indicated in the figure.