Here you can download an example of a LRC-TriCEPS report where we identified the target of a ligand of interest. In this specific experiment we had three treatment arms, the ligand of interest, a positive control (Transferrin) and a negative control (bovine serum albumin). As there were three treatment arms, three comparisons of the relative abundance of the identified proteins are shown.
During the pretest experiments https://www.dualsystems.com/pretest-triceps-service-kit/ it was evaluated that the expression of the unknown targets are dependent on activation of T-cells, therefore the cells were activated accordingly. Further, by the use of Flow-TriCEPS (https://www.dualsystems.com/pretest-triceps-service-kit/ it was determined that the best way to add the ligands to the cells in this specific case is pH 7.4, for 30 minutes at 22°C. Alternatively, if unknown targets are expressed in strongly adherent cells that are not suited for flow cytometry, binding can also be determined using the Tamra-TriCEPS kit and services https://www.dualsystems.com/tamra-triceps/
In our report, the identified target proteins are further described in more details, showing exactly which peptides of the target proteins were found and their relative abundance in each treatment arm. When the report is delivered, we schedule a conference call to explain all the data and answer questions you might have.
Further, with the Target confirmation https://www.dualsystems.com/target-confirmation/ we offer a service to knock down the identified targets to provide a first validation.
Download the whole LRC-TriCEPS identification report here as pdf.
For further information or to obtain a quote for a target identification experiment on living cells please contact email@example.com