Identification of cell surface proteins (surfaceome)
by TriCEPS
TriCEPS-Surfaceome on primary human T-cells
Why is the surfaceome (sum of all proteins at the cell surface) important?
a) Cell communication: Detects and transmits cell signals
b) Cell differentiation and activation: Changes its protein expression pattern depending on internal and external signals
c) Cell protection: Keeps cell integrity and provides a boundry
d) Cell migration: Leads the way by communicating with its surrounding
e) Immunity: Key for identification of self and foreign and the diseased state of a cell
For all these functions the surface proteins (surfaceome) are key.
The surfaceome content differs among cell types and changes during developmental and disease states. Therefore, it contains unique Biomarkers that can be used to distinguish cellular phenotypes and disease states. These properties along with the fact that cell surface proteins are readily available make the surfaceome a rich source of phenotypic, diagnostic, prognostic and therapeutic targets that can be used in a variety of fields including oncology, immunology and stem cell research
Using our TriCEPS platform technology we can identify the surface proteins of any given cell and compare the quantitative changes between two cellular states.
As an example experiment, we show the quantitative changes taking place on primary human CD4+ T cells activated by CD3, CD28 and IL-2 for 3 and 11 days compared to naïve T cells.
Figure 1: As an example experiment, we show the quantitative changes taking place on primary human CD4+ T cells activated by CD3, CD28 and IL-2 for 3 days and 11 days compared to naïve T cells. The TriCEPS surfaceome workflow was conducted to identify quantitative changes of surface proteins on the naïve and activated primary human T cells.
Roughly 600 membrane associated proteins (surfaceome) were identified for the primary T cells; approximately 150 and 200 proteins showed differential expression after 3 and 11 days of activation respectively when compared to naïve T-cells.
Contact form
Please fill out all mandatory (*) fields.
Volcano plots depicting the comparison between naive and activated T cells
Data is shown at the protein level and proteins were annotated using the human proteome database from Uniprot. Identified proteins were filtered and only membrane associated and/or secreted proteins were retained. Results are presented in the format of a volcano plot; Y axis = -Log10 (adj. p-value), X-axis = Log2 fold change compared to the other sample.
Proteins in the green space showed an increase in abundance upon T cell stimulation whereas proteins in the blue space were more abundant on naïve T cells.
Known T cell surface activation and proliferation markers, including receptor proteins (e.g. TFR1 and IL2RA (CD25)) co-stimulatory molecules (e.g. ICOS and TNR4 (OX40)), adhesion proteins (e.g. ICAM1) and interleukines (e.g. IL16) were found to be differentially abundant upon stimulation
Left plot: proteins known to change in abundance upon activation
Right plot: proteins constitutively expressed
Figure 2: Expression profiles of known T cell surface activation and proliferation Biomarkers across naïve and activated T cells
Contact form
Please fill out all mandatory (*) fields.
LRC-TriCEPS / HATRIC-LRC Publications
Concerning the LRC-TriCEPS or HATRIC-LRC platforms.
Acidity changes immunology: a new VISTA pathway
Nature Article VISTA interaction with PSGL-1 identified by LRC-TriCEPS
Anti-VISTA antibody that inhibits Vista function and blocks interaction with PSGL-1 and VSIG3 proteins slows tumor growth
Phage resistance at the cost of virulence
PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1008032 October 7, 2019
White Paper Ligand-Receptor Identification Methodologies Details Matter
Validation of extracellular ligand–receptor interactions by Flow‑TriCEPS
Cardiac Targeting Peptide, a Novel Cardiac Vector: Studies in Bio-Distribution, Imaging Application, and Mechanism of Transduction
Leukocyte differentiation by histidine-rich glycoprotein/stanniocalcin-2 complex regulates murine glioma growth through modulation of anti-tumor immunity
Glycomics and Proteomics Approaches to Investigate Early Adenovirus–Host Cell Interactions
HATRIC-based identification of receptors for orphan ligands
Staphylococcal Superantigens Use LAMA2 as a Coreceptor GPCT signaling To Activate T Cells
Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma
Phenotypic screening—the fast track to novel antibody discovery
Identification of Putative Receptors for the Novel Adipokine CTRP3 Using Ligand-Receptor Capture Technology
Serum stimulation of CCR7 chemotaxis due to coagulation factor XIIa-dependent production of high-molecular-weight kininogen domain 5
Laminin targeting of a peripheral nerve-highlighting peptide enables degenerated nerve visualization
Identification of cell surface receptors for the novel adipokine CTRP3
Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
- Received:
- Accepted:
- Published online:
A Mass Spectrometric-Derived Cell Surface Protein Atlas
Protter
Ligand-based receptor identification on living cells and tissues using TRICEPS
Flex your TRICEPS
Interested in a surfaceome project?
Please contact us!
Customers Testimonials – LRC-TriCEPS Service
Testimonials from our customers who have used the LRC-TriCEPS technology – in collaboration with Dualsystems Biotech AG.
LRC-TriCEPS customers worldwide
Over 200 satisfied customers from 28 countries.
- Extracellular proteins
- Peptide ligands
- Antibodies
- Engineered affinity binders
- Viruses
- Small molecules
- Extracellular proteins
- Peptide ligands
- Antibodies
- Engineered affinity binders
- Viruses