Why is the surfaceome (sum of all proteins at the cell surface) important?
a) Cell communication: Detects and transmits cell signals
b) Cell differentiation and activation: Changes its protein expression pattern depending on internal and external signals
c) Cell protection: Keeps cell integrity and provides a boundry
d) Cell migration: Leads the way by communicating with its surrounding
e) Immunity: Key for identification of self and foreign and the diseased state of a cell
For all these functions the surface proteins (surfaceome) are key.
The surfaceome content differs among cell types and changes during developmental and disease states. Therefore, it contains unique Biomarkers that can be used to distinguish cellular phenotypes and disease states. These properties along with the fact that cell surface proteins are readily available make the surfaceome a rich source of phenotypic, diagnostic, prognostic and therapeutic targets that can be used in a variety of fields including oncology, immunology and stem cell research
Using our TriCEPS platform technology we can identify the surface proteins of any given cell and compare the quantitative changes between two cellular states.
As an example experiment, we show the quantitative changes taking place on primary human CD4+ T cells activated by CD3, CD28 and IL-2 for 3 and 11 days compared to naïve T cells.