Use of LRC-TriCEPS to characterize the surfaceome of extracellular vesicles (EV)
April 04, 2025
Intro
Presented at the annual meeting of the International Society for Extracellular Vesicles (ISEV) 2025 Annual Meeting in April PDF for downloadAbstract
Advancing EV Research: Novel Technique for EV's Surfaceome Profiling.
The extracellular vesicle (EV) surfaceome plays a crucial role in vesicle targeting, uptake, and functional interactions. However, its characterization remains challenging due to EV heterogeneity and small size, limiting conventional proteomics approaches. In this study, we employed a chemoproteomic approach originally designed for the identification of cell surface proteins in living cells, and adapted it for the untargeted profiling of EV surfaceome. This methodology uses selective enrichment of N-, C-, and Oglycosylated proteins, which are ubiquitously present on the plasma membrane of living cells and, by extension, on the EV surface.Author
Zuzana Vince Kazmerova, Jakub Janko and Lucia BalazovaFooter
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