How we support you
Dualsystems Biotech is a service provider for custom proteomics for industry and academia. We are a biotech company specialized in elucidating protein-protein interaction in living cells focusing at the cell membrane. We identify the targets of your extracellular ligands (small molecules, peptides, proteins, antibodies, ADC’s, Fc-fusion proteins, and viruses) using the ligand-receptor capture LRC-TriCEPS and the HATRIC-LRC platforms. During the interaction of your ligand of interest and its unknown targets and off-targets the cells are still alive and the unknown targets are in their natural microenvironment. There is no need to genetically modify the cells and any cell type (cell lines or primary cells) can be used.
We elucidate in a 3-step approach the mode of action of your ligand of interest.
With Flow-TriCEPS (Pretest) we developed a tool to visualize the binding of your ligand on cells using flow cytometry. Alternatively, with TAMRA-TriCEPS we can visualize the binding of TriCEPS coupled ligand to the cells also by confocal microscopy. During those pretests, we identify the best binding conditions (time, temperature, pH, co-factors, and cell activation) for your ligand of interest without the need of a detection antibody. Further, we provided Flow-TriCEPS as a kit so that you can test in your functional assays if your TriCEPS coupled ligand is still active. Flow-TriCEPS and TAMRA-TriCEPS couple the ligand by using primary amines (N-Term and Lysines) and since it contains a visualization function, binding can be detected by flow cytometry or confocal microscopy.
Once the optimal binding conditions are identified the next step can be initiated. In the identification studies, we use TriCEPS or HATRIC coupled ligand to treat the cells. The cells are mildly oxidized so that the second arm of TriCEPS / HATRIC binds with a covalent bond to the glycans of the targets/off-targets. The third arm of TriCEPS is used to enrich the surface proteins. Proteins are identified through bottom-up proteomics, relatively quantified and compared to one or several controls (we advise to use two controls). The most enriched proteins are the target candidates for the respective ligand. In general, we expect a low number of target candidates (less than 5).
In the third step, the identified target candidates can be downregulated using siRNA and if a reduced binding of the ligand of interest is observed with Flow-TriCEPS or TAMRA-TriCEPS compared to the wild type cells the identified candidates are confirmed.
Identify the mode of action using the TriCEPS platforms
Areas of expertise
Specialized in identifying targets and off targets on living cells to elucidate the mode of action.
Experts in proteomics, LC-MS/MS analyses, and cell biology.