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LRC-blau-TriCEPS-02

Dualsystems Biotech AG

Dualsystems Biotech AG


Dualsystems Biotech is a service provider for custom proteomics for industry and academia. We are a biotech company specialized in elucidating protein-protein interaction in the living cells especially at the cell membrane. We identify the targets of your extracellular ligands using the ligand-receptor capture LRC-TriCEPS™ technology. During the interaction of your ligand of interest and its unknown targets and off-targets the cells are still alive and the unknown targets are in their natural microenvironment.

Further, with Flow-TriCEPS™ (Prestest TriCEPS™) we provide a tool to use in flow cytometry to identify the best binding conditions for your ligand of interest without the need of a detection antibody. Flow-TriCEPS can also be used in functional assays to test if TriCEPS coupled ligand is functionally active. Flow TriCEPS couples the ligand by using primary amines (N-Term and Lysines) and since it contains a biotin, can be detected by a streptavidin fluorophore conjugate using flow cytometry.

Flow TriCEPS Services/Kit



pretest-triceps-service-kit-yellow-flurescence1

Detection of the binding of your ligand to the cells using TriCEPS V2.0

Identification of the targets using TriCEPS V2.0 in or at the cell membrane of your ligand (peptide, protein, Antibody, ADC or other primary amine containing molecules) requires that the cells used are expressing the unknown targets. TriCEPS V2.0 is the ideal tool to screen cell lines or primary cells to identify the best cells to use later in the LRC-TriCEPS target identification experiments. Dualsystems offers these pretests for cell selection as service and kit. Needed for the experiment is the ligand of interest and the cell lines/ primary cells that are expected to express the unknown target. There is no need for a ligand specific antibody.

Used for:

  • Screening of different cells
  • Cell selection
  • Positive control selection
  • Negative control selection
  • Functional Assays with TriCEPS coupled ligand
  • Flow Cytometric (FACS) reagent to analyze protein/peptide binding to cells

A conceptual new approach for ligand-receptor capture on the living cell

Ligand receptor capture LRC-TriCEPS™ is a conceptually new technology to analyse the protein interaction of your biologics. We can identify the targets of your protein, antibody and peptide at the cell surface. Dualsystems offers the LRC-TriCEPS™ technology as a service for fee model to the industry and academia. The technology enables the unbiased identification of cell surface targets and off-targets on the living cells without genetic manipulation. Ligand-receptor capture technology can be used for many types of orphan ligands, such as peptides, proteins, antibodies, engineered affinity binders but also for viruses.

Orphan ligands:

  • Extracellular proteins
  • Peptide ligands
  • Antibodies
  • Engineered affinity binders
  • Viruses

Did you see our Video Interview about LRC-TriCEPS™ Services, ligand-receptor capture technology on the living cell?

LRC-TriCEPS and Flow-TriCEPS Flyer

Do you want to do functional pretests with your ligand coupled to LRC-TriCEPS™?

Dualsystems Biotech partners with IBR Inc. to validate the workflow of the CaptiRec Technology

Dualsystems Biotech AG Press Release

Dualsystems Biotech partners with IBR Inc. to validate the workflow of the CaptiRec Technology

Schlieren, Switzerland, January 2014: – Dualsystems Biotech AG has announced it will collaborate with another Swiss laboratory, Institute for Biopharmaceutical Research (IBR Inc.) to validate the CaptiRec Triceps/Ligand Receptor Capture Technology. This technology is used to identify the Cell Membrane Receptors on Living Cells.
The partnership will combine the respective strengths of Dualsystems, a leading service provider for protein interaction at the cell membrane and IBR Inc., a GLP-compliant contract research organization specializing in cell-based assays

Read more

Sitemap

Sitemap

  • Home | Dualsystems Biotech is a provider of custom proteomics services for industry and academia, specializing in ligand-receptor capture, affinity purification/mass spectrometry analysis of protein complexes and stable cell line generation.In addition, Dualsystems provides kits and reagents for a range of research applications, including protein expression, protein-protein interaction and protein activity measurement.
  • LRC-TriCEPS Services | LRC-TriCEPS™ ligand-receptor capture Services
    • Overview | TriCEPS™-based identification of ligand-receptor interactions
    • Principle | Dualsystems identify binding partners for Ligand-receptor interaction
    • Literature | TriCEPS-based ligand-receptor capture
  • Affinity and receptor expression | Assessment of the limits of the LRC-TriCEPS™ technology
  • Cell Types | Living cell types used with LRC-TriCEPS
  • Example of LRC-TriCEPS applied to human primary T-cells
  • Example of a LRC-TriCEPS experiment
  • Flow (Pretest) TriCEPS™ Kit |
  • News | Get here the latest news about Dualsystems Biotech custom screening services and products.
  • Blog | Blog article from Dualsystems.
  • Events | Events, trade fairs, partnering events Dualsytems plans to attend.
  • Company | Dualsystems Biotech is a provider of custom services to identify protein-protein interactions, including yeast two-hybrid services and our proprietary DUALmembrane and DUALhunter technologies for the identification of interactions involving membrane proteins and transcription factors. In addition, we provide kits and reagents for a range of research applications, including protein expression, protein purification and protein activity measurement.
  • Management of Dualsystems | Find more information about the Dualsystems Biotech management team.
  • Scientific Board | Find here more information about the Dualsystems Biotech scientific advisory board.
  • Investors | Are you interested in investment opportunities with Dualsystems Biotech?
  • Career | Are you interested in working for Dualsystems Biotech?
  • Contact | Get in contact with Dualsystems Biotech. Contact us by email, telephone or our online contact form and we will come back to you within 24 hours!
  • Bioinformatics tools | Bioinformatics tools

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LRC-blau-TriCEPS-02

Dualsystems Biotech AG

Dualsystems Biotech AG


Dualsystems Biotech is a service provider for custom proteomics for industry and academia. We are a biotech company specialized in elucidating protein-protein interaction in the living cells especially at the cell membrane. We identify the targets of your extracellular ligands using the ligand-receptor capture LRC-TriCEPS™ technology. During the interaction of your ligand of interest and its unknown targets and off-targets the cells are still alive and the unknown targets are in their natural microenvironment.

Further, with Flow-TriCEPS™ (Prestest TriCEPS™) we provide a tool to use in flow cytometry to identify the best binding conditions for your ligand of interest without the need of a detection antibody. Flow-TriCEPS can also be used in functional assays to test if TriCEPS coupled ligand is functionally active. Flow TriCEPS couples the ligand by using primary amines (N-Term and Lysines) and since it contains a biotin, can be detected by a streptavidin fluorophore conjugate using flow cytometry.

Flow TriCEPS Services/Kit



pretest-triceps-service-kit-yellow-flurescence1

Detection of the binding of your ligand to the cells using TriCEPS V2.0

Identification of the targets using TriCEPS V2.0 in or at the cell membrane of your ligand (peptide, protein, Antibody, ADC or other primary amine containing molecules) requires that the cells used are expressing the unknown targets. TriCEPS V2.0 is the ideal tool to screen cell lines or primary cells to identify the best cells to use later in the LRC-TriCEPS target identification experiments. Dualsystems offers these pretests for cell selection as service and kit. Needed for the experiment is the ligand of interest and the cell lines/ primary cells that are expected to express the unknown target. There is no need for a ligand specific antibody.

Used for:

  • Screening of different cells
  • Cell selection
  • Positive control selection
  • Negative control selection
  • Functional Assays with TriCEPS coupled ligand
  • Flow Cytometric (FACS) reagent to analyze protein/peptide binding to cells

A conceptual new approach for ligand-receptor capture on the living cell

Ligand receptor capture LRC-TriCEPS™ is a conceptually new technology to analyse the protein interaction of your biologics. We can identify the targets of your protein, antibody and peptide at the cell surface. Dualsystems offers the LRC-TriCEPS™ technology as a service for fee model to the industry and academia. The technology enables the unbiased identification of cell surface targets and off-targets on the living cells without genetic manipulation. Ligand-receptor capture technology can be used for many types of orphan ligands, such as peptides, proteins, antibodies, engineered affinity binders but also for viruses.

Orphan ligands:

  • Extracellular proteins
  • Peptide ligands
  • Antibodies
  • Engineered affinity binders
  • Viruses

Did you see our Video Interview about LRC-TriCEPS™ Services, ligand-receptor capture technology on the living cell?

LRC-TriCEPS and Flow-TriCEPS Flyer

Do you want to do functional pretests with your ligand coupled to LRC-TriCEPS™?

Sitemap

Sitemap

  • Home | Dualsystems Biotech is a provider of custom proteomics services for industry and academia, specializing in ligand-receptor capture, affinity purification/mass spectrometry analysis of protein complexes and stable cell line generation.In addition, Dualsystems provides kits and reagents for a range of research applications, including protein expression, protein-protein interaction and protein activity measurement.
  • LRC-TriCEPS Services | LRC-TriCEPS™ ligand-receptor capture Services
    • Overview | TriCEPS™-based identification of ligand-receptor interactions
    • Principle | Dualsystems identify binding partners for Ligand-receptor interaction
    • Literature | TriCEPS-based ligand-receptor capture
  • Affinity and receptor expression | Assessment of the limits of the LRC-TriCEPS™ technology
  • Cell Types | Living cell types used with LRC-TriCEPS
  • Example of LRC-TriCEPS applied to human primary T-cells
  • Example of a LRC-TriCEPS experiment
  • Flow (Pretest) TriCEPS™ Kit |
  • News | Get here the latest news about Dualsystems Biotech custom screening services and products.
  • Blog | Blog article from Dualsystems.
  • Events | Events, trade fairs, partnering events Dualsytems plans to attend.
  • Company | Dualsystems Biotech is a provider of custom services to identify protein-protein interactions, including yeast two-hybrid services and our proprietary DUALmembrane and DUALhunter technologies for the identification of interactions involving membrane proteins and transcription factors. In addition, we provide kits and reagents for a range of research applications, including protein expression, protein purification and protein activity measurement.
  • Management of Dualsystems | Find more information about the Dualsystems Biotech management team.
  • Scientific Board | Find here more information about the Dualsystems Biotech scientific advisory board.
  • Investors | Are you interested in investment opportunities with Dualsystems Biotech?
  • Career | Are you interested in working for Dualsystems Biotech?
  • Contact | Get in contact with Dualsystems Biotech. Contact us by email, telephone or our online contact form and we will come back to you within 24 hours!
  • Bioinformatics tools | Bioinformatics tools