Glycomics and Proteomics Approaches to Investigate Early Adenovirus–Host Cell Interactions

May 07, 2018


Lisa Lasswitz, Naresh Chandra, Niklas Arnberg, Gisa Gerold jmb Journal of Molecular Biology, Received 15 February 2018, Revised 24 April 2018, Accepted 30 April 2018, Available online 7 May 2018.


Adenoviruses as most viruses rely on glycan and protein interactions to attach to and enter susceptible host cells. The Adenoviridae family comprises more than 80 human types and they differ in their attachment factor and receptor usage, which likely contributes to the diverse tropism of the different types. In the past years, methods to systematically identify glycan and protein interactions have advanced. In particular sensitivity, speed and coverage of mass spectrometric analyses allow for high-throughput identification of glycans and peptides separated by liquid chromatography. Also, developments in glycan microarray technologies have led to targeted, high-throughput screening and identification of glycan-based receptors. The mapping of cell surface interactions of the diverse adenovirus types has implications for cell, tissue, and species tropism as well as drug development. Here we review known adenovirus interactions with glycan- and protein-based receptors, as well as glycomics and proteomics strategies to identify yet elusive virus receptors and attachment factors. We finally discuss challenges, bottlenecks, and future research directions in the field of non-enveloped virus entry into host cells.


Lisa Lasswitz1Naresh Chandra2,3Niklas Arnberg2,3Gisa Gerold1,2,4
Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, 30625 Hannover, Germany
Department of Clinical Microbiology, Virology, Umeå University, SE-90185 Umeå, Sweden
Molecular Infection Medicine Sweden (MIMS), Umeå University, SE-90185 Umea, Sweden
Wallenberg Centre for Molecular Medicine (WCMM), Umeå University, SE-90185 Umea, Sweden