Direct identification of ligand-receptor interactions on living cells and tissues

September 16, 2012


Nature Biotechnology 30, 997–1001 doi:10.1038/nbt.2354 – ReceivedAccepted Published online


Here we describe our LRC- TRICEPS platform to identify targets on living cells. We validated this ligand-based, receptor-capture (LRC) technology using different peptides, proteins, antibodies, biologics and viruses. This technology enables the identification of receptors for many types of ligands under near-physiological conditions and without the need for genetic manipulations.


Andreas P Frei, Ock-Youm Jeon, Samuel Kilcher, Hansjoerg Moest, Lisa M Henning, Christian Jost, Andreas Plückthun, Jason Mercer, Ruedi Aebersold, Erick M Carreira & Bernd Wollscheid