Reference

Nature Biotechnology 30, 997–1001 (2012) doi:10.1038/nbt.2354 – Received06 April 2012 Accepted 08 August 2012 Published online 16 September 2012

Abstract

Here we describe our LRC- TRICEPS platform to identify targets on living cells. We validated this ligand-based, receptor-capture (LRC) technology using different peptides, proteins, antibodies, biologics and viruses. This technology enables the identification of receptors for many types of ligands under near-physiological conditions and without the need for genetic manipulations.

Author

Andreas P Frei, Ock-Youm Jeon, Samuel Kilcher, Hansjoerg Moest, Lisa M Henning, Christian Jost, Andreas Plückthun, Jason Mercer, Ruedi Aebersold, Erick M Carreira & Bernd Wollscheid