cDNA library construction
High complexity cDNA library construction
High representation of 5' ends
Guaranteed coverage of tissue complexity
High average insert sizes
Constructed in a vector of your choice
Construction process
- Isolation of total or polyA+ RNA
- Quality control on Experion chip
- 1st cDNA generation and size fractionation
- 2nd strand cDNA generation on two independent size pools
- Quality control on Experion chip
- Directional ligation into appropriate vector
- Amplification of primary library
Specifications
- Special 5' adaptor annealing procedure preserves full-length cDNAs
- Average insert sizes > 1.5 kb
- High complexities (2x10E6 - 5x10E7)
- Cloned into DUALmembrane, DUALhunter and DUALhybrid library vectors, or a vector of your choice
- Average turnaround time 2 months
- You receive primary glycerol stocks and purified plasmid DNA
