CaptiVate technology
Technological Benefits
Physiological expression levels
- Single copy integration at defined site ensures physiological expression levels of the bait
Unparalleled sensitivity and specificity
- High speed, single-step purification retains transient interactors
- Analysis on highly sensitive LTQ Orbitrap
- Quantitative analysis of multiple replicates and data filtering against protein frequency library removes > 95% of all background
High reproducibility
- Greater than 95% overlap between replicate samples
- Intensity plot of identified peptides from two replicate experiments (pearson correlation =0.9)
Co-purification of membrane protein
- Optimized buffer sets ensure excellent coverage of membrane protein baits and interactors
