Vector

Description

Yeast marker

E. coli marker

Map

Sequence

pNCW

Type II bait vector (N-terminal fusion)
cDNAs are expressed as fusions to the C-terminus of the LexA-VP16-Cub reporter cassette (e.g. LexA-VP16-Cub-BAIT). Suitable for cloning type II transmembrane proteins as baits.

LEU2

Kan

pCCW

Type I bait vector (C-terminal fusion)
cDNAs are expressed as fusions to the N-terminus of Cub-LexA-VP16. Expression is driven by the weak CYC1 promoter.

LEU2

Kan

pCCW-SUC

Type I bait vector (C-terminal fusion)
Contains a signal sequence derived from the invertase (SUC2) gene, followed by a multiple cloning site and Cub-LexA-VP16. Use pCCW-SUC if your bait is a type I mammalian transmembrane protein.

LEU2

Kan

pCCW-STE

Type I bait vector (C-terminal fusion)
Contains the N-terminal 15 amino acids from the yeast Ste2 protein, followed by a multiple cloning site and Cub-LexA-VP16. Addition of the Ste2 sequence may enhance expression of certain baits in yeast. Expression is driven by the weak CYC1 promoter.

LEU2

Kan

pDSL-Nx

Library vector (NubG-cDNA orientation)
cDNAs or cDNA libraries are expressed as fusions to the C-terminus of NubG. Expression is driven by the weak CYC1 promoter.

TRP1

Amp

pDL2-Nx

Library vector (NubG-cDNA orientation)
cDNAs or cDNA libraries are expressed as fusions to the C-terminus of NubG. Expression is driven by the strong ADH1 promoter.

TRP1

Amp

pDL2xN

Library vector (cDNA-NubG orientation)
cDNAs or cDNA libraries are expressed as fusions to the N-terminus of NubG. Expression is driven by the strong ADH1 promoter.

TRP1

Amp

pDL2xN-SUC

Library vector (cDNA-NubG orientation)
Contains a signal sequence derived from the invertase (SUC2) gene, followed by a multiple cloning site and NubG. Use pDL2xN-SUC to clone type I mammalian transmembrane proteins as fusions to the SUC2 leader sequence. Expression is driven by the strong ADH1 promoter.

TRP1

Amp

pDL2xN-STE

Library vector (cDNA-NubG orientation)
Contains the N-terminal 15 amino acids from the yeast Ste2 protein, followed by a multiple cloning site and NubG. Addition of the Ste2 sequence may enhance expression of certain preys in yeast. Expression is driven by the strong ADH1 promoter.

TRP1

Amp

pCCW-Alg5

Control bait
Expresses a fusion of the entire open reading frame of the yeast resident ER protein Alg5 to Cub-LexA-VP16. Expression is driven by the weak CYC1 promoter.

LEU2

Kan

pAlg5-NubI

Control prey
Expresses a fusion of the entire open reading frame of the yeast resident ER protein Alg5 to the HA tag and NubI (wild type Nub). Expression is driven by the strong ADH1 promoter.

TRP1

Amp

pAlg5-NubG

Control prey
Expressing a fusion of the entire open reading frame of the yeast resident ER protein Alg5 to the HA tag and NubG. Expression is driven by the strong ADH1 promoter.

TRP1

Amp