Vector

Order number

Description

Yeast marker

E. coli marker

Map

Sequence

pLexA-N

P03105

Bait vector for expression of cDNAs fused to the C-terminus of bacterial LexA . Use this vector if you want to construct a bait for screening in the yeast two-hybrid system.

TRP1

Kan

pLexA-C

P03106

Inverse bait vector for expression of cDNAs fused to the N-terminus of bacterial LexA. Use this vector if you want to screen a bait whose function/folding depends on a free N-terminus.

TRP1

Kan

pGAD-HA

P03103

Library vector based on the popular pGAD series. This vector expresses cDNAs as fusions to the C-terminus of the GAL4 activation domain.

LEU2

Amp

pY3H

P03104

Bridging vector for the expression of a third protein partner in the yeast two-hybrid system. Compatible with all our yeast two-hybrid and DUALmembrane vectors.

URA3

Amp

pLexA-p53*

P03112

Control vector for the DUALhybrid system. A fragment of human p53 is expressed as a fusion to LexA. Positive control when cotransformed with pACT-largeT.

TRP1

Kan

pLexA-laminC*

P03113

Control vector for the DUALhybrid system. A fragment of human laminC is expressed as a fusion to LexA. Negative control when cotransformed with pACT-largeT.

TRP1

Kan

pACT-largeT*

P03114

Control vector for the DUALhybrid system. A fragment of SV40 large T antigen is expressed as a fusion to the GAL4 activation domain.

LEU2

Amp