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DUALhunter vectorsVector maps and sequences for the DUALhunter system
Vector | Order number | Description | Yeast marker | E. coli marker | Map | Sequence | pDHB1 | P0350 | DUALhunter bait vector
cDNAs are inserted between the Ost4 membrane anchor and the Cub-LexA-VP16 reporter cassette. | LEU2 | Kan | 
Map (PDF/109 KB) | 
Complete sequence | pPR3-N | P03234 | DUALhunter prey vector
cDNAs are fused to the C-terminal end of the NubG module. Suitable for construction of cDNA libraries. | TRP1 | Amp |
Map (PDF/112 KB) |
Complete sequence | pDHB1-largeT | P03503 | DUALhunter control vector
Expresses SV40 largeT antigen fused to the Ost4 anchor and Cub-LexA-VP16. | LEU2 | Kan |
Map (PDF/102 KB) |
Complete sequence | pDSL-p53 | P03504 | DUALhunter control vector
Expresses p53 as a fusion to NubG. To be used as positive control in combination with pDHB1-largeT. | TRP1 | Amp |
Map (PDF/102 KB) |
Complete sequence | pAI-Alg5 | | Control prey vector
Expresses a fusion of the entire open reading frame of the yeast resident ER protein Alg5 to the HA tag and NubI (wild type Nub). Expression is driven by the strong ADH1 promoter. | TRP1 | Amp |
Map (PDF/109 KB) |
Complete sequence | pDL2-Alg5 | | Control prey vector
Expresses a fusion of the entire open reading frame of the yeast resident ER protein Alg5 to the HA tag and NubG. Expression is driven by the strong ADH1 promoter. | TRP1 | Amp |
Map (PDF/109 KB) |
Complete sequence |
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