Vector  

Description

Map  

Sequence  

pTFB1

Type II bait vector (N-terminal fusion)
cDNAs are expressed as fusions to the C-terminus of the LexA-VP16-Cub reporter cassette (e.g. LexA-VP16-Cub-BAIT). Suitable for cloning type II transmembrane proteins as baits.

pCMBV4

Type I bait vector (C-terminal fusion)
cDNAs are expressed as fusions to the N-terminus of Cub-LexA-VP16. Expression is driven by the weak CYC1 promoter.

pAMBV4

Type I bait vector (C-terminal fusion)
cDNAs are expressed as fusions to the N-terminus of Cub-LexA-VP16. Expression is driven by the strong ADH1 promoter.

pTMBV4

Type I bait vector (C-terminal fusion)
cDNAs are expressed as fusions to the N-terminus of Cub-LexA-VP16. Expression is driven by the strong TEF1 promoter.

pDSL-Nx

Library vector (NubG-cDNA orientation)
cDNAs or cDNA libraries are expressed as fusions to the C-terminus of NubG. Expression is driven by the weak CYC1 promoter.

pDL2-Nx

Library vector (NubG-cDNA orientation)
cDNAs or cDNA libraries are expressed as fusions to the C-terminus of NubG. Expression is driven by the strong ADH1 promoter.

pDL2xN  

Library vector (cDNA-NubG orientation)
cDNAs or cDNA libraries are expressed as fusions to the N-terminus of NubG. Expression is driven by the strong ADH1 promoter.

pDL2xN-SUC

Library vector (cDNA-NubG orientation)
Contains a signal sequence derived from the invertase (SUC2) gene, followed by a multiple cloning site and NubG. Use pDL2xN-SUC to clone type I mammalian transmembrane proteins as fusions to the SUC2 leader sequence. Expression is driven by the strong ADH1 promoter.

pDL2xN-STE

Library vector (cDNA-NubG orientation)
Contains the N-terminal 15 amino acids from the yeast Ste2 protein, followed by a multiple cloning site and NubG. Addition of the Ste2 sequence may enhance expression of certain preys in yeast. Expression is driven by the strong ADH1 promoter.

pCCW-Alg5

Control bait
Expresses a fusion of the entire open reading frame of the yeast resident ER protein Alg5 to Cub-LexA-VP16. Expression is driven by the weak CYC1 promoter.

pAlg5-NubI

Control prey
Expresses a fusion of the entire open reading frame of the yeast resident ER protein Alg5 to the HA tag and NubI (wild type Nub). Expression is driven by the strong ADH1 promoter.

pAlg5-NubG 

Control prey
Expressing a fusion of the entire open reading frame of the yeast resident ER protein Alg5 to the HA tag and NubG. Expression is driven by the strong ADH1 promoter.