Product number

Strain

Description

Genotype

Datasheet

P04005

NMY51

NMY51 is the screening strain of choice for the DUALmembrane 3 system and for LexA-based yeast two-hybrid systems. Two different growth markers and a lacZ reporter ensure the highest possible stringency in a library screen, resulting in fewer false positives. NMY51 has a ura3 genotype. This strain is a component of the DUALmembrane kit 3.

MATa his3delta200 trp1-901 leu2-3,112 LYS2::(lexAop)4-HIS3 ura3::(lexAop)8-lacZ (lexAop)8-ADE2 GAL4

P04001

DSY-1

General reporter strain for use with the DUALhybrid or DUALmembrane systems. DSY-1 can be used with any LexA-based yeast two-hybrid system and is also suitable for use with the original DUALmembrane system. This strain has been replaced by NMY51.

MATa his3delta200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3 URA3::(lexAop)8-lacZ GAL4

No datasheet available

P04004

NMY32

NMY32 is an optimized screening strain for yeast two-hybrid and DUALmembrane systems. Two growth markers and a lacZ reporter ensure the highest possible stringency in a library screen, resulting in fewer false positives. This strain has been replaced by NMY51.

MATa his3delta200 trp1-901 leu2-3,112 LYS2::(lexAop)4-HIS3 URA3::(lexAop)8-lacZ (lexAop)8-ADE2 GAL4

No datasheet available

P04002

DSY-2

This strain has been replaced by NMY61.

MATalpha his3delta200 trp1-901 leu2-3,112 ade2 URA3::(lexAop)8-lacZ GAL4

No datasheet available

P04006

NMY61

Mating partner for reporter strain NMY51.

MATalpha his3delta200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3
ura3::(lexAop)8-lacZ (lexAop)8-ADE2 GAL4)

P04003

DSY-5

DSY-5 is a general purpose strain for expression of recombinant proteins in yeast. Deletion of the two major vacuolar proteases Pep4 and Prb1 ensures good yield and little non-specific degradation when purifying proteins from yeast. Can be used in combination with our general purpose yeast expression vectors.

MATalpha leu2 trp1 ura3-52 his3::GAL1-GAL4 pep4 prb1-1122

P04007

DSY-6

DSY-6 has been designed for expression of recombinant proteins in yeast. Good growth characteristics ensure high cell densities both in synthetic defined (SD) and rich (YPD) medium. Deletion of prb1 encoding the vacuolar proteinase B (yscB), pep4 encoding the vacuolar aspartyl protease (proteinase A) and prc1 encoding the vacuolar carboxypeptidase Y (proteinase C) ensures minimal protein degradation during cell lysis and protein extraction.

MATa leu2 trp1delta63 ura3-52 prb1-22 pep4-3 prc1-407