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View Questions by Category
About Dualsystems (8)
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Antibodies (1)
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cDNA library construction (1)
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DUALhybrid (44)
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DUALmembrane (39)
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Protein expression systems (4)
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Protein extraction (2)
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Protein refolding (9)
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Working with yeast (14)
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Most Popular Questions
How can I test whether my protein cloned into my prey vector is localized in the membrane? Is there an antibody against NubG?- What does the genotype of your strains mean?
- What is a false positive?
- The beta-galactosidase assay protocol didn't work as my yeast two-hybrid clones didn't really turn blue. What do you advise?
- What is the genotype and reference for DSY-5/JEL1?
- How does DUALSYSTEMS eliminate false positives?
- Are yeast two-hybrid screens reproducible?
- I want to publish the results of a DUALhybrid screen. How do I acknowledge Dualsystems?
- Where can I find maps and sequences of DUALmembrane or DUALhybrid vectors?
- Which library (NubG-X or X-NubG) should I use for a DUALmembrane screen?
Recent Entries
- Isolation of total RNA from Escherichia coli
- What is contained in the DUALrefold buffers?
- Do the yeast expression vectors pTEF-MF and pGAL-MF contain an epitope tag for detection?
- What is the length of the mating factor alpha leader in pTEF-MF and pGAL-MF?
- I am unable to detect my bait by Western blot. What can I do?
- Do the DUALXtract buffers show absorbance at 280 nm?
- What is the formulation of Reagent C in the DUALrefold kit?
- Some basic information on replicating plasmids in yeast
- How should I proceed with functional assays after protein refolding?
- Is refolding at lower temperatures better?
