A novel approach to analyse extracellular cell signaling by discovering cell surface receptors and off-targets for a ligand of interest on the living cell: label ligand with the LRC-TriCEPS™crosslinker, incubate with living cells or tissue expressing target receptor(s), purify receptor crosslinked peptides, analyse by LC-MS/MS. This new technology was invented by Andreas Frei and Bernd Wollscheid from the ETH Zurich who published the technology in Nature Biotechnology (Frei et al.(2012) Nature Biotechnology 30:997-1001).
- Extracellular proteins
- Peptide ligands
- Engineered affinity binders
CaptiVate Shotgun Proteomics Services using LC-MS/MS
- Identification of protein complexes and protein interactions in mammalian cells
- Profiling of signaling pathways
- analysis of post-translational modifications