HTX kit
Quickly measure beta-galactosidase levels with a minimum of hands-on time: less than 15 minutes for 96 clones!
Easy semi-quantitative scoring by eye or using a flatbed scanner
Quantitative measurements possible
Cheaper than ONPG or filter-lift assays
One kit is sufficient for 4x96 assays
Technology information
Quick and easy quantitative analysis of interactors from yeast two-hybrid and DUALmembrane screens!
In a typical yeast two-hybrid or DUALmembrane screen, dozens or even hundreds of putative interactors are often identified.
Commonly used assays to detect beta-galactosidase activity, such as the filter lift assay or the ONPG assay, are either not quantitative or very cumbersome to use. In contrast, the HTX kit yields semi-quantitative measurements with minimal handling.
Literature citing the use of the HTX assay kit
Möckli et al. (2004)
Saydam et al. (2007)
Kit contents
Component | Amount |
One-Step lysis and assay reagent (buffer salts and detergents) | 50 ml |
Dye stock solution (X-Gal substrate) | 250 µl |
Ordering information
Order number | Product | Size |
P01002 | HTX beta-galactosidase assay kit | 1 kit |
