Highly efficient refolding of denaturated cytosolic proteins
Test 10 different refolding conditions using the DUALrefold column set
- Once the optimal refolding conditions have been identified, large scale refolding can be performed
Efficient refolding of denatured cytosolic proteins
Often, proteins can only be purified from total cellular extracts using denaturing conditions. But even when applying mild conditions, some proteins can only be purified in a denatured state. This is particularly true for larger proteins with complicated structures.
The principle of protein refolding is based on the following parameters:
- Different proteins require different conditions to fold
- Conditions that stabilize the folding intermediates often help to yield correctly folded proteins
- Columns completely remove denaturants
The DUALrefold system integrates all these three factors and provides a refolding kit which is both very efficient and easy to use.
DUALrefold soluble protein refolding kit
Using the DUALrefold soluble protein refolding kit, optimal protein folding conditions can be identified by a simple bench-top centrifugation and incubation procedure. No tedious dialysis or high ratio dilution is needed. No special equipment or protein folding experience is required to use the folding kit. About 0.06 - 0.12 mg of inactive protein can be used per refolding assay.
Small scale refolding columns
Small scale DUALrefold protein folding columns are designed for small-scale (0.6 - 1.2 mg) preparative protein folding experiments after the optimal folding conditions have been identified. The column set includes reagents and 10 identical protein folding spin-columns.
Large scale refolding columns
Large scale DUALrefold protein folding columns are designed for preparative protein folding experiments (5 -10 mg protein) after the optimal folding conditions have been identified. The column set includes 4 identical preparative protein folding columns and reagents.
Component | Amount |
DUALrefold soluble protein refolding columns | 10 spin columns |
Ordering information
Order number | Product | Quantity |
P07201 | DUALrefold soluble protein refolding kit | 10 spin column sets |
P07301 | DUALrefold soluble protein refolding column (small) #1 | 10 spin columns |
P07302 | DUALrefold soluble protein refolding column (small) #2 | 10 spin columns |
P07303 | DUALrefold soluble protein refolding column (small) #3 | 10 spin columns |
P07304 | DUALrefold soluble protein refolding column (small) #4 | 10 spin columns |
P07305 | DUALrefold soluble protein refolding column (small) #5 | 10 spin columns |
P07306 | DUALrefold soluble protein refolding column (small) #6 | 10 spin columns |
P07307 | DUALrefold soluble protein refolding column (small) #7 | 10 spin columns |
P07308 | DUALrefold soluble protein refolding column (small) #8 | 10 spin columns |
P07309 | DUALrefold soluble protein refolding column (small) #9 | 10 spin columns |
P07310 | DUALrefold soluble protein refolding column (small) #10 | 10 spin columns |
P07401 | DUALrefold soluble protein refolding column (large) #1 | 4 columns |
P07402 | DUALrefold soluble protein refolding column (large) #2 | 4 columns |
P07403 | DUALrefold soluble protein refolding column (large) #3 | 4 columns |
P07404 | DUALrefold soluble protein refolding column (large) #4 | 4 columns |
P07405 | DUALrefold soluble protein refolding column (large) #5 | 4 columns |
P07406 | DUALrefold soluble protein refolding column (large) #6 | 4 columns |
P07407 | DUALrefold soluble protein refolding column (large) #7 | 4 columns |
P07408 | DUALrefold soluble protein refolding column (large) #8 | 4 columns |
P07409 | DUALrefold soluble protein refolding column (large) #9 | 4 columns |
P07410 | DUALrefold soluble protein refolding column (large) #10 | 4 columns |
