Find novel interaction partners for your integral membrane protein!
With the DUALmembrane kit you can screen full-length, integral membrane proteins and membrane-associated proteins against a cDNA library of your choice to identify novel interaction partners of your protein of interest.
Features of the DUALmembrane kit 3
- Screen full-length integral membrane proteins for interactors in vivo
- Protein interactions are detected under physiological conditions at the membrane
- Novel interactors are identified through screening of cDNA libraries
- Easy subcloning of full-length cDNA inserts using Sfi I technology
- NMY51 reporter strain with improved stringency, resulting in fewer false positives
- Trial size HTX beta-galactosidase assay kit included (2x 96 reactions)
DUALmembrane starter package
Take advantage of the DUALmembrane starter package to get quickly started: it includes the DUALmembrane kit 3 and two additional kits for easy quantitative determination of β-galactosidase levels and efficient library scale yeast transformation.
The DUALmembrane starter package includes:
- DUALmembrane kit 3
- Standard size HTX beta-galactosidase assay kit (4x 96 reactions)
- DS Yeast transformation kit
Examples of membrane proteins used in the DUALmembrane system 
Kit Contents
Component | | Amount |
pBT3-N | Type II bait vector | 5 µg |
pBT3-C | Type I bait vector | 5 µg |
pBT3-SUC | Type I signal sequence bait vector | 5 µg |
pBT3-STE | Type I leader sequence bait vector | 5 µg |
pPR3-N | prey vector | 5 µg |
pPR3-C | prey vector | 5 µg |
pPR3-SUC | Signal sequence prey vector | 5 µg |
pPR3-STE | Leader sequence prey vector | 5 µg |
pCCW-Alg5 | control bait | 5 µg |
pAI-Alg5 | control prey | 5 µg |
pDL2-Alg5 | control prey | lyophilized |
NMY51 | yeast reporter strain | yeast |
Ordering information
P01001 | DUALmembrane kit 3 |
P09901 | DUALmembrane starter package |
