The DUALhybrid kit is based on the classical yeast two-hybrid system and includes a range of improvements to facilitate yeast two-hybrid screening.

 

Applications of the DUALhybrid kit include:

• Screening of soluble proteins for novel binding partners
• Screening of protein fragments or domains for novel binding partners
• Interaction domain mapping and mapping of binding interfaces by site-directed mutagenesis
• Detection of multimeric complexes (yeast three-hybrid)

 

Compared to traditional yeast two-hybrid kits, the DUALhybrid kit features many improvements:

• Complete kit for all types of yeast two-hybrid studies, including complex interaction studies (yeast three-hybrid)
• Choice of two bait vectors for maximum flexibility and decreased chances of self-activating baits
• Triple reporter strain NMY51 ensures an optimal screening stringency
• Complete set of controls: pLexA-p53, pLexA-lamin C and pACT-largeT control vectors
• Detailed manual and online help

Bait vectors, prey vectors and controls for the DUALhybrid system are also available separately.

Ordering information

Order number	Product	Quantity
P01004	DUALhybrid kit	1 kit
P03103	pGAD-HA prey vector	5 µg lyophilized
P03104	pY3H bridging vector	5 µg lyophilized
P03105	pLexA-N bait vector	5 µg lyophilized
P03106	pLexA-C bait vector	5 µg lyophilized
P03107	DUALhybrid control set	5 µg lyophilized

 

>> Vector maps and sequences >> DUALhybrid vector selection guide