Find novel interaction partners for transcriptionally active proteins!
With the DUALhunter kit you can screen full-length soluble (cytosolic or nuclear) proteins against a cDNA library of your choice to identify novel interaction partners of your protein of interest.
Features of the DUALhunter kit:
- Quickly screen your protein of interest for novel interactors in vivo
- No need to truncate your protein of interest or to select subdomains
- Screen transcriptionally active proteins or self-activating proteins
- Easy subcloning of full-length cDNA inserts using Sfi I technology
- Reporter strain with additional markers and improved stringency, resulting in fewer false positives
Kit Contents
Component | | Amount |
pDHB1 | Bait vector | 5 µg |
pPR3-N | Prey vector | 5 µg |
pDHB1-largeT | Control bait vector | 5 µg |
pDSL-p53 | Control prey vector | 5 µg |
pAI-Alg5 | Control prey | 5 µg |
pDL2-Alg5 | Control prey | 5 µg |
NMY51 | yeast reporter strain | lyophilized yeast |
Ordering information
