DUALmembrane starter kits

Screen full-length integral membrane proteins in vivo
Protein interactions are detected in intact cells
Identify new interactors by cDNA library screening
Compatible with GPCRs, RTKs, ion channels and most other membrane proteins

With every kit you buy, you can choose 1 cDNA library for free!

Click here to check our cDNA library collection

DUALmembrane starter kit N

This kit contains the pBT3-N bait vector. The Cub module is fused to the N-terminus of the protein of interest. Suitable for all type II transmembrane proteins.

DUALmembrane starter kit SUC

This kit contains the pBT3-SUC bait vector. The vector contains an N-terminal cleavable signal sequence and the Cub module is fused to the C-terminus of the protein of interest. Suitable for type I transmembrane proteins.

DUALmembrane starter kit STE

This kit contains the pBT3-STE bait vector. The vector contains an N-terminal leader sequence and the Cub module is fused to the C-terminus of the protein of interest. Suitable for type I and type II transmembrane proteins.

Download the DUALmembrane decision tree

Assemble your kit individually!

1. Select your bait vector
2. Choose a cDNA library from our collection
3. The customized kit is assembled by us
4. You will receive your product within days!

Kit contents

Contents	DUALmembrane starter kit N	DUALmembrane starter kit SUC	DUALmembrane starter kit STE	Amount

Bait vector	pBT3-N	pBT3-SUC	pBT3-STE	5 ug

Library vector	pPR3-N	pPR3-N	pPR3-N	5 ug

cDNA library	cDNA library of your choice	cDNA library of your choice	cDNA library of your choice	60 ug

Control vectors	pOst1-NubI pTSU2-APP pNubG-Fe65	pOst1-NubI pTSU2-APP pNubG-Fe65	pOst1-NubI pTSU2-APP pNubG-Fe65	5 ug each

Reporter strain	NMY51	NMY51	NMY51	Stab culture

Accessory kits	Yeast trans- formation kit HTX Beta-gal assay kit	Yeast transfor- mation kit HTX Beta-gal assay kit	Yeast transfor- mation kit HTX Beta-gal assay kit	1 kit each

Ordering information

DUALmembrane starter kits N

Order number	DUALmembrane starter kit N plus cDNA library:
P01201	Mouse adult whole brain / NubG-x
P01202	Jurkat T cells, unstimulated / NubG-x
P01203	Mouse adult heart / x-NubG
P01204	Mouse adult heart / NubG-x
P01205	Human adult kidney / x-NubG
P01207	Arabidopsis thaliana / NubG-x
P01208	HeLa cell line / NubG-x
P01209	Human embryonal brain / NubG-x
P01210	Human adult colon / NubG-x
P01211	Caenorhabditis elegans adult / NubG-x
P01212	Human adult liver / NubG-x
P01213	Human adult brain / NubG-x
P01214	Caenorhabditis elegans eggs / NubG-x
P01215	Drosophila melanogaster whole embryo (16 hours) / NubG-x
P01216	Mouse adult spleen / x-NubG
P01217	Human adult kidney / NubG-x
P01218	Human adult brain / x-NubG
P01219	LNCaP cell line / NubG-x
P01220	Drosophila melanogaster / NubG-x
P01221	Human adult lung / NubG-x
P01222	Mouse adult kidney / NubG-x
P01223	Mammary epithelial cells / NubG-x
P01224	Rat neonatal cardiomyoctes / NubG-x
P01225	Mouse whole embryo, 11 days / NubG-x
P01226	Medicago nodules / x-NubG
P01227	Medicago nodules / NubG-x
P01228	Saccharomyces cerevisiae / NubG-x
P01229	Mouse whole embryo normalized / x-NubG
P01230	Human spleen normalized / NubG-x
P01231	Normalized mouse brain / NubG-x
P01232	Normalized human fetal brain / NubG-x

DUALmembrane starter kits SUC

Order number	DUALmembrane starter kit SUC plus cDNA library:
P01301	Mouse adult whole brain / NubG-x
P01302	Jurkat T cells, unstimulated / NubG-x
P01303	Mouse adult heart / x-NubG
P01304	Mouse adult heart / NubG-x
P01305	Human adult kidney / x-NubG
P01307	Arabidopsis thaliana / NubG-x
P01308	HeLa cell line / NubG-x
P01309	Human embryonal brain / NubG-x
P01310	Human adult colon / NubG-x
P01311	Caenorhabditis elegans adult / NubG-x
P01312	Human adult liver / NubG-x
P01313	Human adult brain / NubG-x
P01314	Caenorhabditis elegans eggs / NubG-x
P01315	Drosophila melanogaster whole embryo (16 hours) / NubG-x
P01316	Mouse adult spleen / x-NubG
P01317	Human adult kidney / NubG-x
P01318	Human adult brain / x-NubG
P01319	LNCaP cell line / NubG-x
P01320	Drosophila melanogaster / NubG-x
P01321	Human adult lung / NubG-x
P01322	Mouse adult kidney / NubG-x
P01323	Mammary epithelial cells / NubG-x
P01324	Rat neonatal cardiomyoctes / NubG-x
P01325	Mouse whole embryo, 11 days / NubG-x
P01326	Medicago nodules / x-NubG
P01327	Medicago nodules / NubG-x
P01328	Saccharomyces cerevisiae / NubG-x
P01329	Mouse whole embryo normalized / x-NubG
P01330	Human spleen normalized / NubG-x
P01331	Normalized mouse brain / NubG-x
P01332	Normalized human fetal brain / NubG-x

DUALmembrane starter kits STE

Order number	DUALmembrane starter kit STE plus cDNA library:
P01401	Mouse adult whole brain / NubG-x
P01402	Jurkat T cells, unstimulated / NubG-x
P01403	Mouse adult heart / x-NubG
P01404	Mouse adult heart / NubG-x
P01405	Human adult kidney / x-NubG
P01407	Arabidopsis thaliana / NubG-x
P01408	HeLa cell line / NubG-x
P01409	Human embryonal brain / NubG-x
P01410	Human adult colon / NubG-x
P01411	Caenorhabditis elegans adult / NubG-x
P01412	Human adult liver / NubG-x
P01413	Human adult brain / NubG-x
P01414	Caenorhabditis elegans eggs / NubG-x
P01415	Drosophila melanogaster whole embryo (16 hours) / NubG-x
P01416	Mouse adult spleen / x-NubG
P01417	Human adult kidney / NubG-x
P01418	Human adult brain / x-NubG
P01419	LNCaP cell line / NubG-x
P01420	Drosophila melanogaster / NubG-x
P01421	Human adult lung / NubG-x
P01422	Mouse adult kidney / NubG-x
P01423	Mammary epithelial cells / NubG-x
P01424	Rat neonatal cardiomyoctes / NubG-x
P01425	Mouse whole embryo, 11 days / NubG-x
P01426	Medicago nodules / x-NubG
P01427	Medicago nodules / NubG-x
P01428	Saccharomyces cerevisiae / NubG-x
P01429	Mouse whole embryo normalized / x-NubG
P01430	Human spleen normalized / NubG-x
P01431	Normalized mouse brain / NubG-x
P01432	Normalized human fetal brain / NubG-x