Protein extraction from fungi and bacteria can be challenging, due to the stable cell wall which must be disrupted prior to detergent-assisted protein extraction and solubilization.

The TOTAL protein extraction kit has been specially formulated for efficient extraction and solubilization of total protein mixtures from fungi and bacteria.

After mechanical disruption of the cell wall by glass beads, proteins are solubilized using a complex detergent mix. The use of several detergents ensures uniform solubilization of proteins with widely different characteristics, including strongly hydrophobic proteins, such as integral membrane proteins.

After solubilization, proteins are denatured by addition of sample buffer and loaded onto an SDS-PAGE gel for further analysis.

The kit is recommended for preparation of total protein extracts in DUALhybrid, DUALmembrane and DUALhunter screens and is also compatible with  other yeast two-hybrid systems.

Kit contents