Efficient isolation of membrane proteins from mammalian cells and tissue samples
Simple and rapid procedure isolates high quality membrane and cytoplasmic protein extracts without cross-contamination between the two fractions
The procedure is based on stepwise differential protein solubilization. Treatment with different mild, non-denaturing detergent based buffers selectively separates membrane and membrane-associated proteins from the cytoplasmic fraction.
Cells are first permeabilized with the Cell Permeabilization Buffer to release the cytoplasmic protein fraction. The cell debris is then treated with the Membrane Protein Extraction Buffer which selectively solubilizes the majority of integral and membrane-associated proteins and isolates them as a separate fraction.
The kit is supplied with sufficient reagents for 50 extractions from 5 million cells or 20-40 mg of tissue per experiment.
Component | Amount |
Cell Permeabilization Buffer | 100 ml |
Membrane Protein Extraction Buffer | 50 ml |
Cell Wash Solution | 250 ml |
Order number | Product | Size |
P07115 | DUALXtract total membrane protein extraction kit | 1 kit |
