Technology information

 

Highly efficient extraction of integral membrane proteins

Purification of membrane proteins is a complex,  time consuming process and requires complete extraction and solubilization of the proteins from the membranes of the host cell.

Commonly encountered problems when extracting membrane proteins:

• Inefficient extraction of the protein of interest
• Inactivation of protein function and/or unfolding during extraction

The DUALXtract solutions provide optimal conditions for efficient extraction and stabilization of membrane proteins from the cell membranes by using a variety of non-denaturing detergent and synthetic lipid analogs.

How to use the DUALXtract buffer set to identify optimal extraction conditions

Step 1 Use the DUALXtract buffer set to test 12 different extraction buffers on your membrane protein of interest Step 2 Once you have identified the optimal extraction buffer, use this buffer to perform a large scale extraction Step 3 Continue with downstream work using the extracted membrane protein

Kit contents

Component	Amount
DUALXtract buffer set (trial size)	12 vials 0.5 ml each
DUALXtract buffer set	12 vials   5 ml each

Ordering information

Order number	Product	Size
P07001	DUALXtract buffer set  (trial size)	1 kit
P07002	DUALXtract buffer set	1 kit
P07101	DUALXtract buffer 1	40 ml
P07102	DUALXtract buffer 2	40 ml
P07103	DUALXtract buffer 3	40 ml
P07104	DUALXtract buffer 4	40 ml
P07105	DUALXtract buffer 5	40 ml
P07106	DUALXtract buffer 6	40 ml
P07107	DUALXtract buffer 7	40 ml
P07108	DUALXtract buffer 8	40 ml
P07109	DUALXtract buffer 9	40 ml
P07110	DUALXtract buffer 10	40 ml
P07111	DUALXtract buffer 11	40 ml
P07112	DUALXtract buffer 12	40 ml

Dualsystems Biotech AG | Grabenstrasse 11a | 8952 Schlieren | Switzerland | Phone: +41 (0)44 738 50 00 | Fax: +41 (0)44 738 50 05 | info@dualsystems.com