Mammalian cell total protein extraction kit
Highly effective and convenient total protein extraction from mammalian cultured cells or tissue samples
No sonication and no freeze-thaw cycles are required
Technology information
Highly efficient extraction of total proteins from mammalian cells and tissue
Proteins extracted using this reagent are non-denatured, functionally active and therefore ideal for direct use in many common downstream applications, including 1D and 2D electrophoresis, Western blotting, electrophoretic mobility shift assay (EMSA), immunoprecipitation, affinity purification, enzymatic activity and reporter gene assays. Isolated proteins are compatible with quantification assays such as Bradford, Lowry and the BCA assay. The reagent provided is sufficient for:
- 125 extractions from 100 ยตl of wet cell pellet or
- 500 extractions from 100 mg of tissue
Kit contents
Component | Amount |
Mammalian cell total protein extraction reagent | 250 ml |
Ordering information
Order number | Product | Size |
P07113 | DUALXtract mammalian cell total protein extraction kit | 1 kit |
