Applications

Yeast expression vectors

p427-TEF

High copy yeast expression vector carrying the aminoglycoside phosphotransferase gene for selection in yeast using G418. Inserts are expressed from the strong TEF promoter.

p417-CYC

Low copy yeast expression vector carrying the aminoglycoside phosphotransferase gene for selection in yeast using G418. Inserts are expressed from the weak CYC1 promoter.

 

pTEF-MF

Yeast expression vector for secreted proteins. A strong TEF1 promoter drives constitutive expression of a cDNA fused to the pre-pro leader sequence of mating factor alpha to ensure secretion of the protein product into the medium.

pGAL-MF

Yeast expression vector for secreted proteins. A regulatable GAL-L promoter drives inducible expression of a cDNA fused tothe pre-pro leader sequence of mating factor alpha to ensure secretion of the protein product into the medium.

 

 

 

Order number

Product

Amount

P03301

p427-TEF

5 µg

P03302

p417-CYC

5 µg

P03303

pTEF-MF

5 µg

P03304

pGAL-MF

5 µg

Supporting information

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