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Yeast expression vectorsp427-TEFHigh copy yeast expression vector carrying the aminoglycoside phosphotransferase gene for selection in yeast using G418. Inserts are expressed from the strong TEF promoter. p417-CYCLow copy yeast expression vector carrying the aminoglycoside phosphotransferase gene for selection in yeast using G418. Inserts are expressed from the weak CYC1 promoter.
pTEF-MFYeast expression vector for secreted proteins. A strong TEF1 promoter drives constitutive expression of a cDNA fused to the pre-pro leader sequence of mating factor alpha to ensure secretion of the protein product into the medium. pGAL-MFYeast expression vector for secreted proteins. A regulatable GAL-L promoter drives inducible expression of a cDNA fused tothe pre-pro leader sequence of mating factor alpha to ensure secretion of the protein product into the medium.
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