QuickLyse: rapid autolysis of bacterial strains for recombinant protein purification

The novel QuickLyse vector pEAS-1a provides rapid, temperature-controlled lysis of bacteria expressing your recombinant protein of interest. Just clone your gene into pEAS-1a, transform into an appropriate expression host and induce protein expression, followed by a temperature shift to induce autolysis.  After lysis, the recombinant protein is immediately accessible for downstream purification and analysis.

• Especially suitable for high-throughput protein expression
• Recombinant proteins are purified directly from the culture supernatant
• No need for lysozyme treatment or sonication
• Gentle extraction conditions ensure integrity and activity of the recombinant protein

The QuickLyse system uses the autolytic genes derived from the SRRz cluster of phage Lambda (Kloos et al., 1994). The SRRz genes are under the control of the heat inducible cI857/pR promoter from phage Lambda and the strong rrnB terminator. Shifting cells from 28°C to 38°C induces expression of the SRRz genes, resulting in quick and efficient autolysis of bacterial cells.

The system is compatible with common E. coli expression strains, such as BL21 or  XL1-Blue.

 

 

Order number	Product	Vector map	Vector sequence	Quantity
P08001	pEAS-1a  QuickLyse expression vector			5 µg lyophilized

 

References

Kloos et al. (1994) J. Bacteriol. 176:7352-7361

Xu et al. (2006) Biotechniques 41:319-322