Mammalian expression vectors

Mammalian expression vectors for stable or transient expression of cDNAs in mammalian cells:

Strong CMV promoter for high-level, constitutive expression
HA epitope tag for convenient detection of expression products
Sfi I sites for convenient, directional cloning of full-length cDNAs

Sfi I is a rare cutter enzyme which recognizes the sequence GGCCNNNNNGGCC. The variable core sequence allows the design of multiple Sfi I sites with incompatible overhangs within the same vector.

Dualsystems vectors feature two Sfi I sites with the sequences GGCCATTACGGCC and GGCCGCCTCGGCC, allowing directional cloning of cDNAs. Sfi I sequences are extremely rare in eukaryotic genomes and therefore, the majority of eukaryotic cDNAs can subcloned full-length using Sfi I.

Applications

Expression of recombinant proteins in mammalian cells
Transient and stable expression
Follow-up co-immunoprecipitation studies after DUALhybrid (yeast two-hybrid), DUALmembrane and DUALhunter screens

pHA-MEX

Mammalian expression vector featuring a strong CMV promoter, an N-terminal HA epitope tag for detection of expressed proteins and a NeoR cassette for stable integration of the expression vector into the genome of the host cell. pHA-MEX is suited for both transient overexpression and long-term, stable expression.

pMEX-HA

Mammalian expression vector featuring a strong CMV promoter, a C-terminal HA epitope tag for detection of expressed proteins and a NeoR cassette for stable integration of the expression vector into the genome of the host cell. pMEX-HA is suited for both transient overexpression and long-term, stable expression.

Guide for selection of stable cell lines using pMEX vectors