Mammalian expression vectors for stable or transient expression of cDNAs in mammalian cells:
Strong CMV promoter for high-level, constitutive expression
HA epitope tag for convenient detection of expression products
Sfi I sites for convenient, directional cloning of full-length cDNAs
Sfi I is a rare cutter enzyme which recognizes the sequence GGCCNNNNNGGCC. The variable core sequence allows the design of multiple Sfi I sites with incompatible overhangs within the same vector.
Dualsystems vectors feature two Sfi I sites with the sequences GGCCATTACGGCC and GGCCGCCTCGGCC, allowing directional cloning of cDNAs. Sfi I sequences are extremely rare in eukaryotic genomes and therefore, the majority of eukaryotic cDNAs can subcloned full-length using Sfi I.
pHA-MEX
Mammalian expression vector featuring a strong CMV promoter, an N-terminal HA epitope tag for detection of expressed proteins and a NeoR cassette for stable integration of the expression vector into the genome of the host cell. pHA-MEX is suited for both transient overexpression and long-term, stable expression.
pMEX-HA
Mammalian expression vector featuring a strong CMV promoter, a C-terminal HA epitope tag for detection of expressed proteins and a NeoR cassette for stable integration of the expression vector into the genome of the host cell. pMEX-HA is suited for both transient overexpression and long-term, stable expression.
Guide for selection of stable cell lines using pMEX vectors
Ordering information
| Order number | Product | Amount |
P03401 | pHA-MEX | 5 µg |
P03402 | pMEX-HA | 5 µg |