High level, inducible protein expression in yeast
- Tight regulation and high expression levels
- Grow cells in rich medium, such as YPD
- No medium change required
- Easy protein purification using the popular Strep-tag
The KickStart protein expression kit has been specifically designed for inducible, high-level expression of recombinant proteins in Saccharomyces cerevisiae.
KickStart expression vectors combine two important features for protein expression:
- Inducible ADH2 promoter: The ADH2 promoter is induced when glucose becomes depleted in the medium. During early growth in YPD medium glucose levels are high and the ADH2 promoter is inactive - thereby preventing negative effects on yeast growth.
- Once the cells reach the early stationary phase glucose is depleted from the medium and the ADH2 promoter is induced, producing high amounts of protein.
- KanMX cassette: KickStart™ vectors feature a KanMX cassette, which allows selection with G418 in rich medium. The use of rich medium such as YPD will significantly enhance cell densities and protein yield.
What are the advantages?- High-level, inducible expression
- No addition of reagents or change of medium needed for induction
- Tight regulation of induction allows expression of toxic proteins
- Popular Step-tag for quick and easy protein purification
- Protease deficient expression strain for higher protein yields
- Optimized extraction buffers supplied with the kit
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| KickStart products KickStart kit 
KickStart reagents  |
Expression of beta-galactosidase from pKS1-ST
pKS1-ST-Gal was transformed into the expression strain DSY-5 and grown on YPD plates supplemented with 60 µg/ml G418. Several colonies were mixed and inoculated into YPD supplemented with 60 µg/ml G418. Aliquots were taken at the indicated time points and used to prepare total cell extracts. Beta-galactosidase was detected by Western blotting against the HA epitope tag. The red arrow indicates the time point where glucose in the medium is depleted. | 
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Beta-galactosidase expressed from pKS1-ST is correctly folded and active
Activity of beta-galactosidase in total extracts was assessed at the indicated time points using the HTX beta-galactosidase assay kit (P01002). | 
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KickStart products:
KickStart kit
KickStart reagents
