Technology information

The KickStart protein expression kit has been specifically designed for inducible, high-level expression of recombinant proteins in Saccharomyces cerevisiae.

KickStart expression vectors combine two important features for protein expression:

• Inducible ADH2 promoter: The ADH2 promoter is induced when glucose becomes depleted in the medium. During early growth in YPD medium glucose levels are high and the ADH2 promoter is inactive - thereby preventing negative effects on yeast growth.
• Once the cells reach the early stationary phase glucose is depleted from the medium and the ADH2 promoter is induced, producing high amounts of protein.
• KanMX cassette: KickStart™ vectors feature a KanMX cassette, which allows selection with G418 in rich medium. The use of rich medium such as YPD will significantly enhance cell densities and protein yield.

 

What are the advantages?

• High-level, inducible expression
• No addition of reagents or change of medium needed for induction
• Tight regulation of induction allows expression of toxic proteins
• Popular Step-tag for quick and easy protein purification
• Protease deficient expression strain for higher protein yields
• Optimized extraction buffers supplied with the kit

Kit content

Ordering information

Order number	Product	Size
P01006	KickStart protein expression kit	1 kit
P03305	pKS1 expression vector	5 µg
P03306	pKS1-ST expression vector	5 µg
P03307	pKS1-ST-Gal control vector	5 µg
P03308	pKS2-ST secretion vector	5 µg
P04003	DSY-5 expression strain	lyophilized
P06003	XT extraction buffer & glass beads	50 ml
P01003	DS Yeast transformation kit	1 kit
P06001	Single stranded carrier DNA	3 x 1 ml

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