The DUALXPress protein expression kit contains all vectors, strains and reagents for expression and purification of recombinant proteins from E. coli.

 

Features of the DUALXPress kit:

• Tightly regulated expression
• Increased expression of proteins in the soluble fraction
• One-step purification from crude lysates yields > 95% pure protein
• DualTRAP affinity resin is significantly cheaper than other resins
• DualTRAP resin is highly selective, resulting in fewer background contaminants
• Elution buffer does not interfere with downstream protein quantification
• LYTAG is easily removed by Enterokinase cleavage
• N- and C-terminal LYTAG vectors available

 

DUALXPress kit contents

 

Component	Amount
pALEX2a, b, c expression vectors	5 µg each
REG-12 E. coli expression strain	1 stab
INDUCER solution	25 ml
PROLYSE extraction solution	25 ml
dualTRAP spin columns	25 pcs
Elution buffer	20 ml
AntiLYTAG polyclonal antibody	100 µl

 

 

DUALXPress kit ordering information

 

Order number	Product
P01007	DUALXPress kit