DUALXPress system

The most tightly regulated expression system available

High-level expression of recombinant proteins

Quick and easy purification with the innovative LYTAG

The DUALXPress Protein Expression kit is the natural choice for high level expression and purification of recombinant proteins in E. coli. It incorporates the CASCADE™ Expression System (*) for controlled expression of proteins and the LYTAG (*) purification tag for highly efficient, one-step purification of recombinant proteins.

Proteins cloned into the pALEX2 series of vectors are expressed simply by adding INDUCER solution to the medium. Following induction, recombinant proteins are purified in one step using the dualTRAP affinity resin (Figure 1). Purities of more than 95% can be easily reached in one step due to the exceptionally high selectivity of the dualTRAP resin.

Affinity purification of β-galactosidase. The enzyme was expressed from pALEX2 and affinity purified using dualTRAP resin. Protein yield: 6 mg / 50 ml culture. M: marker, 1: total extract of uninduced cells, 2: total extract after induction, 3: insoluble fraction, 4: soluble fraction, 5: flow-through, 6: washing fraction, 7-9: elution fractions.

Easy expression and purification using the DUALXPress Protein Expression kit

Expression
The CASCADE™ Expression System achieves tight regulation and exceptionally high induction of expression using the sophisticated regulatory circuit of the salicylate-responsive activators of Pseudomonas putida. Upon induction, two independent pathways act synergistically on the Pm promoter to amplify expression of the recombinant protein. The result is a very high signal to noise ratio.
Extraction
PROLYSE solution has been especially formulated to ensure effective extraction of recombinant proteins from E. coli. There is no need for sonification and recombinant proteins are extracted under mild conditions, resulting in preservation of native folding and avoiding aggregation. PROLYSE solution is compatible with the majority of standard purification systems.
Purification
The high selectivity of the LYTAG ensures efficient one-step purification of recombinant proteins with virtually no background contaminants. Elution is easily achieved by addition of elution buffer or by cleavage using enterokinase. The elution buffer does not interfere with standard protein quantitation measurements, making dialysis or buffer exchange unnecessary.

DUALXPress protein expression kit

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