Most tightly regulated expression system available
High-level expression of recombinant proteins
Increased solubiltiy
One-step purification and up to 95% pure protein
Technology information
Easy expression and purification using the DUALXPress Protein Expression kit
Expression
The CASCADE™ Expression System achieves tight regulation and exceptionally high induction of expression using the sophisticated regulatory circuit of the salicylate-responsive activators of Pseudomonas putida. Upon induction, two independent pathways act synergistically on the Pm promoter to amplify expression of the recombinant protein. The result is a very high signal to noise ratio.
Extraction
PROLYSE solution has been especially formulated to ensure effective extraction of recombinant proteins from E. coli. There is no need for sonification and recombinant proteins are extracted under mild conditions, resulting in preservation of native folding and avoiding aggregation. PROLYSE solution is compatible with the majority of standard purification systems.
Purification
The high selectivity of the LYTAG ensures efficient one-step purification of recombinant proteins with virtually no background contaminants. Elution is easily achieved by addition of elution buffer or by cleavage using enterokinase. The elution buffer does not interfere with standard protein quantitation measurements, making dialysis or buffer exchange unnecessary.
Additional technical information
Kit contents
Component | Amount |
pALEX2a expression vector (N-terminal fusion) | 5 µg |
pALEX2-CT expression vector (C-terminal fusion) | 5 µg |
REG-12 E.coli expression strain | 1 stab |
INDUCER solution (1M) | 500 µl |
PROLYSE extraction solution | 12 ml |
dualTRAP spin columns | 10 pcs |
ELUTION buffer (3M) | 500 µl |
Anti LYTAG polyclonal antibody | 100 µl |
WASH buffer | 40 ml |
Order information
Order number | Product | Size |
P01008 | DUALXPress starter kit | 1 kit |
P03601 | pALEX2a expression vector | 5 µg |
P03602 | pALEX2b expression vector | 5 µg |
P03603 | pALEX2c expression vector | 5 µg |
P03604 | pALEX2a,b,c vector set | 5 µg each |
P03605 | pALEX2-CT expression vector | 5 µg |
P04101 | REG-12 E.coli expression strain | 1 stab |
P06101 | PROLYSE extraction solution | 25 ml |
P06102 | PROLYSE extraction solution | 100 ml |
P06103 | dualTRAP affinity resin | 30 ml |
P06104 | dualTRAP affinity resin | 250 ml |
P06105 | dualTRAP spin columns | 10 pcs |
P06106 | dualTRAP spin columns | 25 pcs |
P06107 | dualTRAP spin columns | 100 pcs |
P06108 | dualTRAP multiwell affinity plates | 1 pcs |
P06109 | dualTRAP multiwell affinity plates | 5 pcs |
P06110 | INDUCER solution | 25 ml |
P06111 | Elution buffer | 20 ml |
P06007 | Rabbit polyclonal antiLYTAG antibody | 100 µl |
