The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a great method for preparing proteins for many downstream applications
Each spin column is able to concentrate and desalt up to 50 ug of acidic or basic proteins
The QuickSpin protein concentration, buffer exchange & desalting kit is designed for concentrating small volumes of dilute protein solutions, for buffer exchange, and for removing different types of salts from protein samples.
The kit utilizes spin columns, which bind the protein of interest if it retains a net positive charge. Nonspecifically bound materials such as salts are washed from the column and the specific protein is eluted into a small volume of elution buffer. The process results in an effective concentration and desalting of the protein. Each spin column is able to concentrate and desalt up to 50 µg of acidic or basic protein.
Component | Amount |
Column Equilibration and Wash Buffer (Acidic) | 30 mL |
Column Activation and Wash Buffer (Basic) | 30 mL |
pH Binding Buffer (Acidic) | 3 mL |
pH Binding Buffer (Basic) | 3 mL |
Elution Buffer | 4 mL |
Neutralizer | 1 mL |
Micro Spin Columns | 25 |
Collection Tubes | 25 |
Elution tubes (1.7 mL) | 25 |
Ordering information
Order number | Product | Quantity |
P07701 | QuickSpin protein concentration, buffer exchange & desalting kit | 1 kit |
