More than 95% of detergents from protein samples removed
Easy to handle Micro Spin Columns guarantee a high protein recovery
The purified protein can be used in many downstream applications such as mass spectrometry, SDS-PAGE, proteolytic digestion, isoelectric focussing, x-ray cristallography, and NMR spectroscopy
The kit is able to remove all types of detergents including ionic, non-ionic and zwitterionic detergents. It is designed to remove detergents in protein solutions either in their free form or bound form, as when complexed with the protein. Purification is based on spin column chromatography.
The QuickSpin detergent removal kit designed to remove detergents in solutions of acidic or basic proteins. Detergents are removed by phase partitioning with ion-exchange chromatography, and the general principle for removal applies to different classes of detergents.
The kit utilizes spin columns, which bind the protein of interest if it retains a net positive charge. Non-specifically bound proteins and detergents are washed from the column and the specific protein is eluted into a specially formulated elution buffer, removing the detergent from the protein sample. Each column will remove detergent from up to 50 µg of acidic or basic protein.
Component | Amount |
Column Activation and Wash Buffer (Acidic) | 8 mL |
Column Activation and Wash Buffer (Basic) | 8 mL |
pH Binding Buffer (Acidic) | 4 mL |
pH Binding Buffer (Basic) | 4 mL |
Elution Buffer | 4 mL |
Neutralizer | 1 mL |
Micro Spin Columns | 25 |
Collection Tubes | 25 |
Elution tubes (1.7 mL) | 25 |
Ordering information
Order number | Product | Quantity |
P07702 | QuickSpin detergent removal kit | 1 kit |
