DUALhybrid (yeast two-hybrid) cDNA libraries

High quality cDNA libraries for the DUALhybrid system/ Compatible with classical (Gal4-based) yeast two-hybrid systems

Article number: DUALhybrid cDNA libraries Size: 60 µg / 200 µg Storage temperature: -20°C Shipping temperature: RT

Applications

Discovery of novel protein interactions by DUALhybrid screening
Compatible with all classical Gal4-based yeast two-hybrid systems
cDNAs are cloned into pGAD-HA or pGAD-DS (N-terminal GAL4-fusion)

Article Number	Product name	Description
P12101	Mouse embryo 11d cDNA library (pGAD-DS)	Mouse cDNA library for screening of cytosolic and nuclear bait proteins
P12117	Mouse cortical neurons cDNA library (pGAD-HA)	Mouse cDNA library for screening of cytosolic and nuclear bait proteins
P12112	Mouse adult ovary cDNA library (pGAD-HA)	Mouse cDNA library for screening of cytosolic and nuclear bait proteins
P12107	Mouse adult heart cDNA library (pGAD-HA)	Mouse cDNA library for screening of cytosolic and nuclear bait proteins
P12401	Normalized yeast two hybrid Mouse Brain cDNA library	Mouse cDNA library for screening of cytosolic and nuclear bait proteins
P12106	Human hepatocellular cell cDNA library (pGAD-HA)	Human cDNA library for screening of cytosolic and nuclear bait proteins
P12102	Human adult colon cDNA library (pGAD-DS)	Human cDNA library for screening of cytosolic and nuclear bait proteins
P12402	Normalized yeast two hybrid Human Fetal Brain cDNA library	Human cDNA library for screening of cytosolic and nuclear bait proteins
P12116	HUVEC cDNA library (pGAD-HA)	Human cDNA library for screening of cytosolic and nuclear bait proteins
P12114	MCF-7S1 (mammary gland adenocarcinoma) cDNA library (pGAD-HA)	Human cDNA library for screening of cytosolic and nuclear bait proteins
P12103	HeLa cell line cDNA library (pGAD-DS)	Human cDNA library for screening of cytosolic and nuclear bait proteins
P12115	Zebrafish PGC 9-12h cDNA library (pGAD-HA)	Zebrafish cDNA library for screening of cytosolic and nuclear bait proteins
P12113	Arabidopsis thaliana floral bud cDNA library (pGAD-HA)	Arabidopsis cDNA library for screening of cytosolic and nuclear bait proteins
P12109	Arabidopsis thaliana cDNA library (pGAD-HA)	Arabidopsis cDNA library for screening of cytosolic and nuclear bait proteins
P12108	Saccharomyces cerevisiae cDNA library (pGAD-HA)	S.cerevisiae cDNA library for screening of cytosolic and nuclear bait proteins
P12111	Chlamydomonas reinhardtii cDNA library, (pGAD-HA)	Chlamydomonas cDNA library for screening of cytosolic and nuclear bait proteins
P12110	Plasmodium falciparum cDNA library (pGAD-HA)	Plasmodium cDNA library for screening of cytosolic and nuclear bait proteins
P12105	Caenorhabditis elegans whole adult cDNA library (pGAD-HA)	C.elegans cDNA library for screening of cytosolic and nuclear bait proteins
P12403	Normalized Universal Arabidopsis Thaliana cDNA library	Arabidopsis cDNA library for screening of cytosolic and nuclear bait proteins
P12404	Normalized human universal yeast two-hybrid cDNA library	Normalized human universa cDNA library for screening of cytosolic and nuclear bait proteins

Full-length, highly representative cDNA libraries for the DUALhybrid system and classical yeast two-hybrid systems.

Our high quality cDNA's are constructed using a brand new technology. We utilize the specific features of MMLV-based reverse transcriptase to create full length enriched double stranded cDNA from total or polyA+ RNA samples. cDNAs are amplified by representational PCR and cloned directionally into the DUALhybrid library vectors for use with Dualsystems' DUALhybrid protein interaction screening kits.

With our extensive know-how in library construction we guarantee highest quality with resonable pricing. Delivered as 60 µg or 200 µg purified plasmid DNA, ready for yeast transformation. 60 µg is sufficient for 2 screens, 200 ug is sufficient for 7 screens.

Delivered as 60 µg or 200 µg purified plasmid DNA, ready for yeast transformation.

60 µg is sufficient for 2 screens, 200 ug is sufficient for 7 screens.

MSDS - DUALhybrid libraries