Expression vectors for Escherichia coli

High-level protein expression in E. coli

Article number: Expression vectors for Escherichia coli Size: 5 µg Storage temperature: RT Shipping temperature: RT

Applications

Tightly regulated expression of recombinant proteins in E. coli
Fermentation studies
Antibiotic-free cultivation using parDE operon

Benefits

Ultra-tight regulation and high levels of expression due to Pm/xylS regulon
Compatible with most expression vectors, including pET, pUC and pBAD series
Wide choice of antibiotic resistance markers
RK2 origin allows vector propagation in more than 60 gram negative species

Article Number	Product name	Description
P03703	pVB3 expression vector	Promoter: Pm/xylS \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: -
P03706	pVB6 expression vector	Promoter: - \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: Chloramphenicol
P03709	pVB9 expression vector	Promoter: - \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: parDE
P03701	pVB1 expression vector	Promoter: Pm/xylS \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: -
P03704	pVB4 expression vector	Promoter: Pm/xylS \| Origin: RK2 \| Resistance 1: Tetracyclin \| Resistance 2: -
P03707	pVB7 expression vector	Promoter: - \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: Tetracyclin
P03702	pVB2 expression vector	Promoter: Pm/xylS \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: -
P03705	pVB5 expression vector	Promoter: Pm/xylS \| Origin: RK2 \| Resistance 1: Kanamycin \| Resistance 2: -
P03708	pVB8 expression vector	Promoter: - \| Origin: RK2 \| Resistance 1: Ampicillin \| Resistance 2: Kanamycin

pVB expression vectors

The VB expression technology comprises the most versatile and sophisticated system available for the production of recombinant proteins. The system combines two unique properties: The highly customizable Pm/ xylS expression system and a broad-host-range RK2 minimal replicon. The VB system is ideal for high-level, low-cost, controllable recombinant protein production in E. coli and other gram negative bacteria.

Increased protein production with alternative production organisms

Numerous bacteria can be used for protein production with the VB vectors, including E. coli, Pseudomonas spp. and Caulobacter crescentus, but also many novel organisms that are promising for protein production. Our expression vectors can be used to easily screen for the perfect organism for production of any protein.

5 µg lyophilized plasmid DNA