Our high quality cDNA's are constructed using a brand new technology. We utilize the specific features of MMLV-based reverse transcriptase to create full length enriched double stranded cDNA from total or polyA+ RNA samples. Differences in length of the 5’ adaptor primer ensure transcription of all three reading frames. Normalization of cDNA is confirmed by qPCR using two high abundant marker genes (Figure 1). Normalized cDNAs are amplified by representational PCR and cloned directionally into the library vector of choice.

With our extensive know-how in library construction we guarantee highest quality with reasonable pricing.