Our high quality cDNA libraries can be used in the classical yeast two-hybrid screening or in the two adapted yeast two-hybrid screening technologies DUALmembrane and DUALhunter.
Our cDNA libraries live up to expectations!
Greater than 1x10E6 independent clones
More than 85% plasmids containing inserts
Average insert size at least 1.2 kb
Our high quality cDNA's are constructed using a brand new technology. We utilize the specific features of MMLV-based reverse transcriptase to create full length enriched double stranded cDNA from total or polyA+ RNA samples. Differences in length of the 5’ adaptor primer ensure transcription of all three reading frames. Normalization of cDNA is confirmed by qPCR using two high abundant marker genes (Figure 1). Normalized cDNAs are amplified by representational PCR and cloned directionally into the library vector of choice.
With our extensive know-how in library construction we guarantee highest quality with reasonable pricing.
- Figure 1: qPCR of normalized and not normalized human spleen cDNA. For Actin, a decrease of 75.8% was reached. For hGAPDH, the decrease was 60.6%. The amount of these two high abundant cDNAs was lowered, increasing the quality of the cDNA library.
Currently available normalized cDNA libraries are listed below. Information about a particular library can be obtained by downloading the appropriate datasheet (PDF format). Please inquire for additional normalized cDNA libraries.
Normalized Yeast two-hybrid (DUALhybrid) cDNA libraries
Libraries are supplied as 200 µg or 60 µg purified plasmid DNA, ready for screening. 200 µg is sufficient for seven library scale screens and 60 µg is sufficient for two library scale screens. cDNAs are cloned into pGAD-HA or pGAD-DS (N-terminal GAL4-fusion)
| Order number | Yeast two-hybrid (DUALhybrid) cDNA libraries | Amount | Datasheet |
P02401 | Normalized mouse brain adult | 200 µg |
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P02402 | Normalized human fetal brain | 200 µg |
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P02403 | Normalized Universal Arabidopsis Thaliana | 200 µg |
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P12401 | Normalized mouse brain adult | 60 µg |
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P12402 | Normalized human fetal brain | 60 µg |
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P12403 | Normalized Universal Arabidopsis Thaliana | 60 µg |
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Normalized DUALmembrane/ DUALhunter cDNA librariesLibraries are supplied as 200 µg or 60 µg purified plasmid DNA, ready for screening. 200 µg is sufficient for seven library scale screens and 60 µg is sufficient for two library scale screens. cDNAs are cloned into pPR3-N (N-terminal NubG-fusion) or pPR3-C (C-terminal NubG-fusion) | |||
| Order number | DUALmembrane/ DUALhunter cDNA libraries | Amount | Datasheet |
P02301 | Norm mouse whole embryo 17 days / x-NubG | 200 µg |
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P02303 | Normalized mouse brain / NubG-x | 200ug |
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P02302 | Normalized human spleen / NubG-x | 200 µg |
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P02304 | Normalized human fetal brain / NubG-x | 200 µg |
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P02305 | Normalized universal arabidopsis / NubG-x | 200 µg |
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P02306 | Normalized mouse brain / x-NubG | 200 µg |
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P12301 | Norm mouse whole embryo 17 days / x-NubG | 60 µg |
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P12303 | Normalized mouse brain / NubG-x | 60 ug |
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P12302 | Normalized human spleen / NubG-x | 60 µg |
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P12304 | Normalized human fetal brain / NubG-x | 60 µg |
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P12305 | Normalized universal arabidopsis / NubG-x | 60 µg |
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P12306 | Normalized mouse brain / x-NubG | 60 µg |
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