Amplifcation of complex cDNA mixtures, for example after reverse transcription of RNA
Multiplex PCR
Amplification of large transcripts (> 2 kb)
Amplification of GC rich templates
Amplify full-length, highly representative cDNA from minute quantities of RNA!
The EasyClone PCR kits contains an optimzed mixture of proofreading polymerases to ensure highly accurate, representational PCRs and preferred amplification of large fragments from complex cDNA mixtures.
Example: Multiplex PCR amplification of 15 templates
15 different templates, ranging in size from 0.6 kb to 3 kb, were amplified in one multiplex reaction using the EasyClone PCR kit. The reaction was carried out in a 20 ul volume using the following parameters: initial denaturation at 94°C for 10s, followed by 10 cycles of 94°C for 30s, 65°C for 30s and 72°C for 3m. 5 ul of the reaction was loaded onto a 1% agarose gel, flanked by size markers.
Component | Amount |
50X Easyclone polymerase mix | 100 µl |
10X EasyClone buffer | 600 µl |
50X dNTP mix (10 mM each) | 120 µl |
Control DNA template | 10 µl |
Control PCR primer mix (10 µM) | 10 µl |
Sterile PCR water | 1.5 ml |
This kit provides reagents for 100 standard PCR reactions
Order number | Product | Size |
P01012 | EasyClone PCR kit | 1 kit |
