Fast and easy synthesis of highly complex, representational cDNAs
High representation of full length transcripts
Directional cloning of cDNAs into DUALmembrane and DUALhybrid vectors
Only small starting amounts of RNA required
EasyClone cDNA synthesis is based on a novel technology utilizing the specific features of MMLV-based reverse transcriptase to create full length enriched double stranded cDNA from total or polyA+ RNA samples. cDNAs are amplified by representational PCR and cloned directionally into DUALmembrane or DUALhybrid library vectors for use with Dualsystems' DUALmembrane and DUALhybrid protein interaction screening kits.
No extensive know-how in library construction is needed - the kit supplies all reagents needed for library construction and the detailed manual will guide you through all steps of construction.
Box 1 | |
Component | Amount |
5X first strand buffer | 80 µl |
DTT (20 mM) | 30 µl |
10X dNTP mix (10 mM each) | 25 µl |
PlugOligo-1 adapter (15 µM) | 25 µl |
CDS-1 adapter (10 µM) | 25 µl |
PlugOligo-3M adapter (15 µM) | 25 µl |
CDS-3M adapter (10 µM) | 25 µl |
EasyClone reverse transcriptase | 20 µl |
IP-solution | 130 µl |
Control RNA template (0.5 µg/µl) | 15 µl |
Sterile RNase free water | 1.8 ml |
Box 2 | |
Component | Amount |
50X EasyClone polymerase mix | 50 µl |
10X EasyClone buffer | 300 µl |
PCR Primer M1 (10 µM) | 100 µl |
50X dNTP mix (10 mM each) | 80 µl |
Sterile RNase free water | 1.8 ml |
Control amplified cDNA sample 1 | 25 µl |
Control amplified cDNA sample 2 | 25 µl |
Order number | Product | Size |
P01010 | EasyClone cDNA library construction kit | 1 kit |
