Premade DUALmembrane, DUALhunter and DUALhybrid cDNA libraries
We supply a wide range of premade cDNA libraries for use with our yeast-based screening systems. All cDNA libraries are delivered as purified plasmid DNA, ready for screening.
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DUALmembrane and DUALhunter libraries» more Information |
- cDNAs are cloned into pPR3-N (N-terminal NubG-fusion) or pPR3-C (C-terminal NubG-fusion)
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DUALhybrid libraries» more Information |
- cDNAs are cloned into pGAD-HA or pGAD-DS (N-terminal GAL4-fusion)
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Normalized cDNA libraries» more Information |
- Normalization removes abundant transcripts
- Decreased rate of false positives in yeast-based screenings
- Increased chance of identifying rare interactors in yeast-based screenings
Do you want to construct your own cDNA library?
Often, commercially available cDNA libraries do not satisfy the needs of a researcher working on a particular tissue or animal model. Yet, constructing your own cDNA library for protein interaction screening is not an easy task.
For this reason, we have designed easy to use kits which help you construct highly complex, full-length cDNA libraries with a minimum of hands-on time. There is no need to be a cDNA library expert - carefully optimized reagents and extensive manuals will help you to construct the cDNA library of your choice in no time.
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EasyClone cDNA library construction kit» more Information |
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EasyClone PCR kit» more Information |
NEW: Kit for construction of normalized cDNA libraries!
- Quick and easy construction of normalized cDNA libraries
- Only small amounts of starting RNA required
- Directional cloning
Normalization is the process of removing abundant RNAs from the starting material. Normalized cDNA libraries contain approximately equal amounts of all RNAs and therefore increase the occurrence of rare proteins. This significantly increases the quality of DUALhybrid (yeast two-hybrid), DUALmembrane and DUALhunter screens.
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EasyClone normalized cDNA library construction package» more Information |
