DUALhybrid services

We strongly believe that we offer the best yeast two-hybrid screening service on the market. The two key factors to our quality are mating (complete coverage of the cDNA library ensures that all possible interactors are tested against your bait) and the use of normalized cDNA libraries (normalized libraries remove many of the false positives normally associated with yeast two-hybrid screens).

Applications

Identification of novel interactors for your protein of interest
Testing of pairwise interactions between a set of known proteins
Identification of interaction domains and motifs
Screening for agents which disrupt protein-protein interactions

Service description

Cloning of cDNA encoding your protein of interest into bait vector
Functional assay to confirm proper expression and localization of bait
Pilot screens to adjust selection conditions
Screening of bait against a cDNA library of your choice
Analysis of all interactors from the screen
Removal of false positives by database filtering
You receive a set of interactors of your protein of interest
Optional: delivery of plasmids, independent confirmation of selected interactors

Key advantages

The most optimized two-hybrid process available guarantees exhaustive screening and recovery of all possible protein interactions
A proprietary functional assay ensures that only fully functional baits are screened
Normalized cDNA librarie s increase your chances of identifying rare interactors and reduce non-specific interactions
Mating-based screens ensure complete cDNA library coverage
No missed interactors: all clones from a screen are analyzed