DUALhunter services

The DUALhunter system is a yeast-based screening system to identify interactions involving strongly acidic and nuclear proteins, such as transcription factors.

These proteins are unsuitable for the yeast two-hybrid system since they autonomously activate transcription and lead to false positive results.

The DUALhunter system overcomes this barrier and allows screening of full-length transcription factors for novel interactors.

Applications

Identification of novel interactors for transcription factors and acidic proteins
Testing of pairwise interactions between a set of known proteins
Screening for agents which disrupt protein-protein interactions

Service description

Cloning of cDNA encoding the protein of interest into bait vector
Functional assay to confirm proper expression and membrane localization of bait
Pilot screens to adjust selection conditions
Screening of bait against a cDNA library of your choice
Analysis of all interactors from the screen
Removal of false positives by database filtering
You receive a set of interactors of your protein of interest
Optional: delivery of plasmids

Key benefits

Investigate protein interactions involving transcription factors and acidic proteins
Tested technology, suitable for virtually all soluble proteins - download our application note
Complete coverage: all interactors are analyzed
Normalized cDNA libraries increase coverage and reduce non-specific interactions