DUALmembrane Plus Screening Services
- Screen full-length integral membrane proteins for novel interaction partners
- Post-translational modifications such as glycosylation and disulfide bond formation are preserved
- Weak or transient interactions can be detected
- Interactions are identified in their natural setting at the membrane
- Analysis of all interactors from the screen included
- No claim on intellectual property of results
Part I: Bait construction and analysis
- Bait cloning
- Functional assay to ensure correct expression and insertion of the bait
- Self-activation test and modulation for optimal screening conditions
Part II: Library screen and interactor analysis
- Library screen against cDNA library of your choice
- Picking of primary clones (up to 384 clones)
- 5’ and 3' end sequencing and sequence filtering
- BLAST analysis of all positive interactors
After the screen, you receive:
- 5' and 3' raw sequences of all positive clones
- Prey identification (BLAST search results)
- Prey interaction domain information
Download DUALmembrane Plus service description
Ordering information
Order number | Custom service |
S021 | Custom DUALmembrane Plus screen |
The DUALmembrane system has been developed by Dualsystems Biotech and is covered by patents owned by or licensed to Dualsystems Biotech. DUALmembrane Plus services are co-marketed with and performed by Hybrigenics S. A., Paris, France.
