How is a custom DUALhybrid screen performed?

Supply us with the cDNA encoding your protein of interest Select a cDNA library to be screened from our collection

Part I Bait cloning and verification

Part II Stringency adjustments (pilot screen) Library screen and selection of interactors Analysis of of up to 96 interactors Complete report Sequences and BLAST results delivered to customer

 

 

 

Why order a custom DUALhybrid screen?

• No need to establish the yeast two-hybrid system in your lab
• An extensively optimized system provides high-quality results in a very short time
• Up to 96 interactors from each screen are analyzed, providing complete coverage
• Interactors analyzed and grouped according to confidence levels
• Ready-to-use results enable you to immediately start downstream experiments

 

 

What about screening quality?

• Over six years of experience, hundreds of screens performed
• Fewer than 1 in a 100 screens yield no results
• Average coverage of cDNA libraries: > 2 times the library complexity
• Average number of primary interactors: 70 clones
• More than 75% of interactors are bait-dependent

 

 

 

Additional information

DUALhybrid screening procedure
List of DUALhybrid cDNA libraries
References
The yeast two-hybrid technology in detail