Applications

Custom DUALhunter Plus screening

DUALhunter Plus Screening Services

  • Screen transcription factors or nuclear proteins for novel interaction partners
  • Screen self-activating proteins or acidic proteins for novel interaction partners
  • Weak or transient interactions can be detected
  • Analysis of all interactors from the screen included
  • No claim on intellectual property of results

 

Part I: Bait construction and analysis

  • Bait cloning
  • Functional assay to ensure correct expression of the bait
  • Self-activation test and modulation for optimal screening conditions

 

Part II: Library screen and interactor analysis

  • Library screen against cDNA library of your choice
  • Picking of primary clones (up to 384 clones)
  • 5’ and 3' end sequencing and sequence filtering
  • BLAST analysis of all positive interactors

 

After the screen, you receive:

  • 5' and 3' raw sequences of all positive clones
  • Prey identification (BLAST search results)
  • Prey interaction domain information

 Download DUALhunter Plus service description

 

 

 

Ordering information

Order number

Custom service

S051

Custom DUALhunter Plus screen

 

 

 

 

 

The DUALhunter system has been developed by Dualsystems Biotech and is covered by patents owned by or licensed to Dualsystems Biotech. DUALhunter Plus services are co-marketed with and performed by Hybrigenics S. A., Paris, France.

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© Dualsystems AG, 2007