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Custom cDNA library servicesWe offer custom cDNA library construction for DUALmembrane, DUALhunter and DUALhybrid cDNA libraries.
Service Procedure
Step 1 - Provide us with cells, tissue or RNA. If you wish to use commercially available RNA as a source for your library, please indicate this to us and we will purchase the RNA for you.
- Choose the appropriate library vector
Step 2 - We construct the cDNA library from total or polyA+ RNA . If you provide tissue or cells, we will isolate total or polyA+ RNA from it (please see the guidelines below for amount and quality required).
Library
| Available vectors
| 1st strand priming
| Starting RNA
| DUALmembrane
NubG-X library
| pPR3-N | oligo dT | Total RNA
PolyA+ RNA
| DUALmembrane X-NubG library | pPR3-C | random hexamers | PolyA+ RNA | DUALhunter library | pPR3-N | oligo dT | Total RNA PolyA+ RNA | DUALhybrid library | pGAD-HA | oligo dT / random hexamers | PolyA+ RNA | Other library | please contact us | | |
Step 3
Quality control is performed. Only cDNA libraries which pass the following criteria are amplified: - Greater than 2x106 independent clones
- More than 85% plasmids containing inserts
- Average insert size at least 1.3 kb
Step 4
You receive the following data and materials: - Library quality control sheet
- At least 500 µg of purified library plasmid - ready to screen!
- Glycerol stocks for later amplification of the library
 Download custom cDNA library service description
Download Tissue, Cell and RNA Requirements Datasheet
Ordering information
Order number | Service | S03 | Custom cDNA library construction |
>> Ordering information |
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