Pathway profiling
Multiplexed analysis of protein complexes
Profiling of signalling pathways in human cells
Applications
Find novel interactors of your protein of interest
Monitor protein interactions in response to drugs or extracellular stimuli
Investigate the dynamics of signalling pathways
Apply CaptiVate to target deconvolution and mode of action finding
Service description
- You supply the cDNA encoding your protein of interest (the bait)
- We generate a stable cell line expressing your tagged bait
- We perform affinity purification of the bait and associated proteins directly from human cells
- The composition of the complex is analyzed by high-sensitivity LC-MS/MS
- Non-specific interactors are removed by filtering against contaminant databases
- You receive a list of all proteins associated with your bait
- Simultaneous analysis of multiple baits allows the characterization of signalling pathways
- Treatment of cells prior to purification allows the investigation of dynamic protein interactions
Key Benefits
- Regulated expression of the bait at physiological levels
- Highly efficient and specific purification of native protein complexes
- Exhaustive analysis of complex partners by LC-MS/MS
- Elimination of non-specific background by database filtering
- Interactors are ranked according to confidence
- Delivery of results within 3 months, at an unbeatable price!
